(Follow-up to the previous question) Which of the following would cause a second mobility shift if added to the same mix next? Group of answer choices glucose lactose allolactose cAMP anti-lac repressor antibody

Answers

Answer 1

Allolactose would cause a second mobility shift in the presence of the lac repressor protein, indicating its ability to bind to the repressor and modulate its interaction with the DNA.

If added to the same mix, the presence of allolactose would cause a second mobility shift.

In the lac operon system, the lac repressor protein binds to the operator region of the DNA, preventing the transcription of the lac genes. This binding is released when allolactose, an isomer of lactose, binds to the lac repressor, causing a conformational change and resulting in the release of the lac repressor from the operator.

When conducting a mobility shift assay, a DNA fragment containing the operator region is mixed with a protein, such as the lac repressor. If a substance, such as allolactose, is added to the mix that can bind to the protein and cause a conformational change or dissociation, it will result in a second mobility shift.

This is because the interaction between the protein and the DNA fragment is altered, leading to a different migration pattern during gel electrophoresis.

Therefore, allolactose would cause a second mobility shift in the presence of the lac repressor protein, indicating its ability to bind to the repressor and modulate its interaction with the DNA.

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Related Questions

mc1r and agouti genes are involved in pigmentation in humans and other animals. a study has shown maternal imprinting of an allele that causes yellow coloration (auv) in mice. the regular allele (a) causes dark coloration and it is not maternally imprinted. what patterns of inheritance should we see in the offspring?

Answers

The MC1R and Agouti genes are responsible for pigmentation in humans and other animals. A study has proven that an allele causing yellow pigmentation (auv) is subject to maternal imprinting in mice, while the regular allele (a) does not go through maternal imprinting and produces dark coloration. In the offspring, we should expect a non-uniform pattern of inheritance, with the maternally inherited auv allele causing yellow pigmentation and the paternally inherited regular allele causing dark pigmentation.

Imprinting refers to the gene expression phenomenon where the genetic material from one of the parents is preferentially silenced. In the case of a maternal imprinting of the auv allele in mice, only the maternally inherited allele will express yellow coloration, while the paternal allele that is inherited by the offspring will have no effect on pigmentation. As a result, the offspring that receive a maternal auv allele will display yellow pigmentation, and those who do not will display the dark coloration associated with the regular allele a.The inheritance pattern will be non-uniform as the phenotype depends on whether the offspring has inherited the maternal allele that is imprinted or the paternal allele.

Therefore, the results will be different if the allele that undergoes imprinting comes from the mother rather than the father, illustrating the importance of epigenetic modifications in gene expression and how these can result in non-Mendelian inheritance patterns.

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What did Chargaff discover while studying bases in the DNA of organisms?
The bases in the DNA of each organism were unique.
The ratios of thymine and adenine were similar, as were the ratios of guanine and cytosine.
The overall percentages of bases were different from one organism to the next.
There were equal amounts of all four bases in every organism, or 25 percent of each base.

Answers

Answer:

The overall percentages of bases were different from one organism to the next.

Answer:

The correct answer is c!:)

Explanation:

Hope this helps!:)

Why was the evolution of cyanobacteria such a significant event in Earth's history?

Answers

Answer:

Cyanobacteria are blue-green algae that produce chlorophyll and undergo photosynthesis. Oxygen, a byproduct of that photosynthesis, was released into the atmosphere, changing it in such a way that it may have allowed the evolution of more complex forms of life.

Explanation:

Right on ED2021 practice, goodluck! :) Happy holidays!

1) Write the characters of 'Penguin' with the help of classification chart.
Classification:- i) kingdom:
ii) Phylum:
iii) Class:
iv) Subkingdom:
Characters:-

Answers

Explanation:

hope help you stay happy

What option would be a good adaptation for an animal in a dense RAINFOREST? *
A. heavy body
B. ability to sprint
C. ability to blend with multiple colors and patterns

Answers

Answer:

c because they need to live instead of dying. bc from other bigger animals they can see it and eventually die so.

This article was about a rare monkey, in Myanmar, called the Snub-Nosed Monkey, caught on camera for the first time. The video was shot from the ground and showed a large group of the Snub-Nosed Monkeys traveling through the trees above. The Snub-Nosed Monkey is known for its unique nose which lifts up making the monkey look like it has two apple seeds on its face as opposed to nostrils. Due to habitat destruction, and them living in small numbers it is difficult to study their habits and such. What is known is that whenever it rains they hide out and put their faces in between their knees. This is so the rain does not get in their noses since it may cause them to sneeze making them more vulnerable to predators. These Monkeys are also endangered this as well is due to habitat destruction caused by illegal lodgers.

Answers

This article is about the Snub-Nosed Monkey, a rare species found in Myanmar, that has been caught on camera for the first time. These monkeys are unique because of their noses which lift up, making them look like they have two apple seeds on their face instead of nostrils. It is difficult to study their habits and behaviors due to their habitat destruction and small population.These monkeys are also endangered due to habitat destruction caused by illegal lodgers.

The Snub-Nosed Monkeys are known to hide out and put their faces in between their knees whenever it rains. This is to prevent rain from entering their noses and causing them to sneeze, making them more vulnerable to predators. These monkeys are also endangered due to habitat destruction caused by illegal lodgers. Due to the habitat destruction caused by humans, their population is decreasing at an alarming rate and it is important to protect these rare species.The article is about the Snub-Nosed Monkey which is a rare species found in Myanmar. Due to habitat destruction and their small population, it is difficult to study their habits and behaviors. Whenever it rains, the monkeys hide out and put their faces in between their knees to prevent rain from entering their noses. These monkeys are also endangered due to habitat destruction caused by illegal lodgers.

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Is there some limit to the height of the flight of stairs used
and the horsepower developed? Explain.

Answers

Answer: Yes

After a certain point you reach a max where it becomes tracked at a different measuring method.

Most carbon dioxide is carried from the body tissues to the lungs _____. Most carbon dioxide is carried from the body tissues to the lungs _____. as bicarbonate ions (HCO3 -) combined with hemoglobin by the trachea as hydrogen ions (H ) dissolved in blood plasma

Answers

Answer:

As  bicarbonate ions

Explanation:

Answer:

it a

Explanation:

yw

PLEASE HELP ITS DUE IN 3 HOURS!! 6. Match the correct definition to each vocabulary term:


Answers

Answer:

In order from top to bottom

C

A

E

D

B

Explanation:

1) Reflection is when a ray hits something reflective then bounces back. For example, light reflects off a mirror, which is why you can see yourself in a mirror.

2) Wavelength measures the size of a wave. It is measured from the crest or trough of one wave to the crest or trough of another.

3) Refraction occurs when waves go through different mediums at different speeds. This is why a straw looks bent when in water.

4) Frequency is a way of measuring the speed of a wave. It shows how many waves pass every unit of time.

5) Absorption means that all of the waves were absorbed into the medium and none of them passed through.

This shows cardiac muscle. This type of muscle is *

Answers

Answer:

Involuntary

Explanation:

Hope that helps ;)

Answer:

Involuntary

Explanation:

Cardiac muscle is also an involuntary muscle, found only in the heart. The cardiac muscle fibers all contract together, generating enough force to push blood throughout the body.

Hydraulic fracturing technology is used to produce nuclear power.
a. True
b. False

Answers

The answer is a.true

Answer:

False

Explanation:

An ocean food chain is shown in the diagram above. Which organism represents the trophic level containing approximately 1% of the initial amount of solar energy acquired by the phytoplankton?​

Answers

I believe the answer is copepods

Sand eels represent the trophic level containing approximately 1% of the initial amount of solar energy acquired by phytoplankton.

What is the food chain?

The food chain is an ecological diagram used to represent the connections between different trophic levels.

In the food chain, energy flows from producers to primary consumers and so on.

It has been shown that approximately 90% of energy is lost in the form of heat for each trophic level.

In conclusion, sand eels represent the trophic level containing approximately 1% of the initial amount of solar energy acquired by phytoplankton.

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Which of the following nutritional components will the nurse encourage a patient to consume as it is needed for erythropoiesis?
a. Vitamin B1
b. Vitamin D
c. Zinc
d. Cobalamin

Answers

The following nutritional component will the nurse encourage a patient to consume as it is needed for erythropoiesis is Cobalamin or Vitamin B12.

What is erythropoiesis?

Erythropoiesis is the process by which red blood cells (erythrocytes) are formed. The body's bone marrow contains stem cells that can develop into red blood cells. The average lifespan of a red blood cell is approximately 120 days before it is broken down and eliminated by the body.

What is Cobalamin or Vitamin B12?

Cobalamin, also known as Vitamin B12, is a water-soluble vitamin that aids in the formation of red blood cells and DNA, as well as the proper functioning of the nervous system. Vitamin B12 is primarily found in animal products, including beef, pork, poultry, eggs, and milk, so vegetarians and vegans may have difficulty obtaining enough vitamin B12 from their diet.A deficiency in vitamin B12 can result in anemia, or a reduction in the number of red blood cells, which can lead to fatigue, weakness, and other symptoms. A patient with a vitamin B12 deficiency may need to take supplements or receive injections in order to replenish their body's stores of this essential nutrient. A nurse would advise the patient to consume cobalamin or vitamin B12 as the nutritional component for erythropoiesis as it is needed for the formation of red blood cells.

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Figure 11-1
In which part of the cell does this process shown in Figure 11-1 take place?

Answers

Answer:

The correct answer is - in the ribosomes.

Explanation:

The translation is the process that takes place in the ribosomes attached to the endoplasmic reticulum where they decode the codons present in the form of the mRNA to the amino acid chain with the help of tRNA.

Ribosomes are called the factory of the protein. It involves triplet code to translate into the amino acid sequences to produce polypeptide chains or protein.

Thus, the correct answer is - in the ribosomes.

Polar bears live in extreme conditions. Describe some of the physical traits that
polar bears have to get what they need and avoid threats in their environment?

Answers

Answer:

Thick fur,soles on their paws, white fur.

Explanation:

They need the thick fur to help them with the cold weather.

soles on their paws help with slippery ice,white fur gives camouflage and they have no natural predators.

What form of electromagnetic radiation do microwaves use?

Answers

Answer:

Microwave ovens work by using 2.45 GHz frequency electromagnetic waves know as microwaves to heat the water in food.

a microwave oven uses microwaves to heat food. Microwaves are a type of wave that are sandwiched between radio waves and infrared radiation on the electromagnetic spectrum.

Explanation:

- Eijiro <3

Blood brings to cells things like ____________________ and __________________.

Answers

Answer:

oxygen and nutrients................

In gout, both serum and synovial fluid will have increased levels of:

Answers

In gout, both serum and synovial fluid will have increased levels of uric acid.

Gout is a type of arthritis characterized by the accumulation of urate crystals in joints, which leads to inflammation and severe pain. Uric acid is a natural byproduct of purine metabolism in the body.

Normally, uric acid is dissolved in the blood and excreted through the kidneys. However, in individuals with gout, there is either an overproduction of uric acid or a decreased ability to excrete it, resulting in elevated levels in the bloodstream.

The excess uric acid can precipitate and form crystals in the synovial fluid, which is the lubricating fluid found in joints. These crystals trigger an inflammatory response, causing pain, swelling, and redness characteristic of gout attacks.

The deposition of urate crystals in the synovial fluid can be confirmed by analyzing the fluid obtained through joint aspiration.

Furthermore, since uric acid is elevated in the bloodstream, it is also reflected in the serum levels. Blood tests can measure the serum uric acid levels to aid in the diagnosis of gout.

Therefore, in gout, both serum and synovial fluid will have increased levels of uric acid, reflecting the underlying metabolic imbalance in purine metabolism.

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A gardener performs experiments on her tomato plants. When graphing her data, which factor is an independent variable?
the number of fruit per plant
the amount of light each plant receives
the height of each plant
the average circumference of each plant’s fruit

Answers

Answer:

The answer is "the amount of light each plant receives

At the time of graphing the data, the factor that should be considered as the independent variable should be the light amount that each plant received.

What is the plant?

The plant should be treated as a living thing that should be grown in the earth and contains the stem, leaves, and roots.

At the time when the gardener performed the experiments on the tomato plants so here, the factor should be the light amount that each plant received.

Therefore, the second option is correct.

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Which of the following human impacts on natural habitats are TRUE? Check all that apply.

Answers

This is very easy but all of the above

Vacuoles are cellular organelles that are found in all plant and fungal cells. In plant cells, vacuoles tend to store large amounts of water, so they can push outward on cell walls and help keep plants upright. In fungal cells, vacuoles often store materials that are toxic to the cell.

According to the above information, how do vacuoles help fungal cells maintain homeostasis?
A.
by storing large quantities of water
B.
by allowing the cells to get rid of waste
C.
by producing more cellular organelles
D.
by helping the cells extract energy from food

Answers

Answer:

D. by helping the cells extract energy from food

Explanation:

Fungi are decomposers so they normally need some sort of food to grow. To get the energy to grow, they extract it from the food. Fungi will get their energy from dead animals, dead plants, etc.

What does the green circle part of the graph represent?

A. Primary succession
B. Secondary succession
C. Pioneer specialist
D. Natural disasters

Answers

Answer:

Natural disaster does the green circle mean

Which choice is involved in both sexual and asexual reproduction?

Answers

Answer:

seeds

Explanation:

Answer:

seeds

Explanation:

I took the test right now k12

Which of the following statements about current theories on global warming is true?
a. Scientists generally agree that global warming is taking place.
b. Some scientists think that we should accelerate global warming.
C. The scientific community disagrees about whether we should take action about global
warming
d. Cutting down trees and burning fossil fuels does not lead to global warming.
Please select the best answer from the choices provided
А A
B
С

Answers

Answer:

A. they pretty much all think there is global warming

Answer:

it is  

a.

Scientists generally agree that global warming is taking place.

Explanation:

edge 2021

How can you call something "alive"? All living things share certain characteristics.

Part A: List the 9 characteristics of life. (1 point)

Part B: Choose 3 of the characteristics of life above. Give an example of each that describes how that characteristic contributes to the organism's survival. (3 points)

Answers

Explanation:

A - response to stimuli, reproduction, respiration (breathing), growth and development, regulation, homeostasis, energy processing, evolution,excretion.

there's more but, I've only mentioned 9.

B -

response to stimuli - by responding to stimuli the organism indicates the presence of nervous system and response to maintain survival.reproduction - by reproducing the organism is promoting the species' survival by maintaining it's presence for generations. respiration / breathing - this characteristic indicates gas exchange in an organism which, suggests metabolic reactions in the organism that provides it with necessary energy.

hope this helps. :)

Decomposers (like bacteria and fungi) are not included in this food web. Explain the role of decomposers in nature. Why are they so important?


I NEED HELP WITH THIS PLEASE!!!!

Answers

Answer:

please give me brainlist and follow

Explanation:

Thanks to decomposers, nutrients get added back to the soil or water, so the producers can use them to grow and reproduce. Most decomposers are microscopic organisms, including protozoa and bacteria. ... Instead, fungi get all their nutrients from dead materials that they break down with special enzymes.

What is the greenhouse effect? How does it occur and why is it important? ps i didnt know what subject it was

Answers

Answer: The absorbed energy warms the atmosphere and the surface of the Earth. This process maintains the Earth's temperature at around 33 degrees Celsius warmer than it would otherwise be, allowing life on Earth to exist. Greenhouse gases keep our planet livable by holding onto some of Earth's heat energy so that it doesn't all escape into space. This heat trapping is known as the greenhouse effect. Just as too little greenhouse gas makes Earth too cold, too much greenhouse gas makes Earth too warm.

Explanation:

Which of the following is NOT one of the Nervous System Functions?. Single choice.
(1 Point)

1.To detect changes and feel sensations

2. To initiate appropriate responses to changes

3. To organize information for immediate use and store it for future use

4. Absorb extra fluid between the cells

Answers

Answer:

4. Absorb extra fluid between the cells

Explanation:

The nervous system (SN) is a complex system that acts to receive sensory information through nerves, transmit this information via the spinal cord and finally process it in the brain. The SN has three main roles: sensory input, processing and motor output. The SN can be divided into the central nervous system (composed of the brain and spinal cord), and the peripheral nervous system (composed of those nerves located outside the brain and spinal cord). Moreover, the extracellular fluid between the cells comprises four types of fluids: 1-intravascular fluid (blood), 2-interstitial fluid, 3--transcellular fluid and 4-cerebrospinal fluid. The excess of these fluids is absorbed by the capillaries of the lymphatic system.

Introduction: In 1915, a German scientist named Alfred Wegener proposed the theory of continental drift. According to this theory, the landmasses once were joined into a supercontinent called Pangaea. The landmasses then slowly drifted to their current positions.


Question: What did Pangaea look like?


If South America were close to Africa. If you look at their coastlines, what do you notice?

Answers

Answer:

Pangaea, which looked like a C, with the new Tethys Ocean inside the C, had rifted by the Middle Jurassic, and its deformation is explained below.

Explanation:

The increased availability of genetically modified food has
A)
made such products commonly acceptable.
B)
eliminated famine in developing nations.
C)
made food cheaper to grow in drought areas.
D)
created controversy in the field of bioethics.

Answers

Answer:

B.

Explanation:

This way poorer countries no longer spend so much for so little.

B
Eliminated famine in developing nations.
Other Questions
Physicians' Hospital has the following balances on December 31, 2021, before any adjustment: Accounts Receivable $52.000; Allowance for Uncollectible Accounts $1,200(credit) On December 31, 2021, Physicians' estimates uncollectible accounts to be 10% of accounts receivable.Required:1. Record the adjusting entry for uncollectible accounts on December 31, 2021. (if no entry is required for a particular transaction/event, select "No Journal Entry Required" in the first account field.) Assume that there are only two people in the world, and each person can produce two products, Bread and Beer. The following table shows Zoe's and lan's production possibility frontiers (per hour). Bread Beer 12 8 Zoe lan 4 4 Zoe has a comparative advantage over lan in the production of Bread. lan has a comparative advantage over Zoe in the production of Beer. Assume that Zoe only produces Bread and lan only produces Beer, and they want to trade afterwards. To benefit both people, the price of Bread (in terms of Beer) needs to be the price of Beer (in terms of Bread) needs to be O a. the price of Bread needs to be between 2/3 Beer and 1 Beer; the price of Beer needs to be between 1 Bread and 5/3 Bread. O b. the price of Bread needs to be between 2/3 Beer and 1 Beer; the price of Beer needs to be between 1 Bread and 3/2 Bread. O C. the price of Bread needs to be between 3/5 Beer and 1 Beer; the price of Beer needs to be between 1 Bread and 3/2 Bread. O d. the price of Bread needs to be between 3/5 Beer and 1 Beer; the price of Beer needs to be between 1 Bread and 5/3 Bread. Suppose you have a clause in a forward purchase contract which gives you a partial timing option: you can buy USD against DEM at a forward rate Ft,t, either at time T, or two months earlier. Right now, you are at the intermediate decision date (T - 2 months). So you have to decide whether to buy now or at T. (a) Assume that, at the beginning of this two-month period, the term structure of compound interest rates is flat for maturities up to one year, with a p.a. DEM interest rate of 10 percent and a USD interest rate of 6 percent. Would you decide to buy the USD now (t = T - 2 months), or would you rather wait? Make this decision in each of the following nine situations regarding the current spot rates St and contractual prices > FT: 1.5 2 2.5 (DEM/USD) Contract price Fto.7: Current spot rate 1.5 0 . 2. 2.5 III 22:05 f Back (b) Repeat (a), but reverse the interest rates. (c) How would your answers change if the clause is modified as follows: if you buy immediately, then the amount of DEM would be discounted at the prevailing DEM rate), while the amount of USD payable would be discounted at the prevailing USD interest rate). You should be able to do this without any computations. In the backward induction solution of a bargaining model with n rounds, the rst proposer gets more than half of the surplusa. trueb. false Suppose you invested $94 in the Ishares High Yield Fund (HYG) a month ago. It paid a dividend of $0.50 today and then you sold it for $95. What was your dividend yield and capital gains yield on the investment? WhyAmazon hasn't started a business here in Pakistan ? Explain inDetail On January 1 of the current year, the Queen Corporation issued 9% bonds with a face value of $54,000. The bonds are sold for $52,380. The bonds pay interest semiannually on June 30 and December 31 and a 'Is deregulation of financial institutions blessing for us? Explain briefly. otto leads a team of workers at an advertising firm. recently, one of his subordinates, lucy, suggested that the team celebrate a major milestone by taking the afternoon off to go to a baseball game together at the company's expense. otto cannot approve this request, since the team's budget for entertainment is not enough to pay for everyone. what should otto say to lucy when he meets with her to discuss her proposal? multiple choice if you'd thought about the outings we've already been on and how expensive baseball tickets are, you probably wouldn't have made this suggestion. i would be happy to listen to any other ideas you have about how to celebrate our team's achievement. thanks for the suggestion, but the answer is no. once i decide on an alternate, less expensive way of celebrating, you'll be the first to know. i like the baseball game idea, however it's just not possible right now. asap thansksContrast ethnocentric, polycentric, and geocentric managers, and give a behavioral example of each. Edit Format Table 12pt Paragraph BIUA 2 T : Duval Company issues four-year bonds with a $111,000 par valueon January 1, 2020, at a price of $106,880. The annual contractrate is 8%, and interest is paid semiannually on June 30 andDecember 31. Use the following information to answer #10 and #11 Custard's Bakery produces a pie that requires 2.6 pounds of fruit per pie. The allowance for waste and spoilage per pie is.2 pounds and .1 pounds, respectively. The purchase price of fruit is $2 per pound, but a 4% discount is usually taken. Freight costs are $.10 per pound, and receiving and handling costs are $.07 per pound. The hourly wage rate is $12.00 per hour, but a raise which will average $.30 will go into effect soon. Payroll taxes are $1.20 per hour, and fringe benefits average $2.40 per hour. Standard production time is 1 hour per pie, and the allowance for rest periods and setup is.2 hours and .1 hours, respectively. The standard direct materials price per pound is: $2.17 $2.09 $2.00 $2.13 What are the general legal requirements in organizing acharitable foundation in the Philippines? movetar inc., of phoenix need a new long distance moving van that cost $53,000 with an estimated lifespan of six years and salvage value of $11,000. movetar can secure a $53,000 loan from a local bank repayable in six equal payments at a 10 percent interest rate. Alternatively, movetar can lease the van for $15,000 annually. if purchased, movetar will incur an annual maintenace cost of $2,000. Assume a straight line depreciation, a tax rate of 40% and a cost of capital (WACC) of 16%Given the above information, should movetar, inc. purchase or lease the van. why? provide quatitative support for your recommendation. (60 POINTS) Go back and read the goals for this lesson on page 1. Form a summary statement for each goal, showing you understand and have met the goals of this lab. Be sure to explain all major concepts and relationships presented in this lab. (3-5 sentences)1: Compare the masses, radii, and densities of terrestrial planets and gas giants.2: Describe the shape of planetary orbits.3: Discover Keplers laws:4: Planets revolve around the Sun in elliptical orbits.5: Planets speed up as they move closer to the Sun and slow down as they move farther away from the Sun.6: The cube of a planets orbital radius is proportional to the square of its period.7: Use Keplers third law to predict a bodys period given its orbital radius. _____is defined as the ratio of the compressor (or pump) work input to the turbine work output. Multiple Choice a.Compression ratio b.Pressure ratio c.Cutoff ratio d.Back work ratio True or False? Our town is growing with a doubling time of 40 years so its population will triple in 120 vears. True False The following inventory transactions took place for Oriole Ltd. for the year ended December 31, 2020:Date Event Quantity Cost/Selling PriceJan 1 opening inventory 25,000 $50.00 Jan 5 sale 6,500 71.00 Feb 15 purchase 32,500 41.25 Mar 10 purchase 9,500 48.00 May 20 sale 41,500 71.00 Aug 22 purchase 14,500 48.50 Sep 12 sale 20,500 71.00 Nov 24 purchase 9,500 58.50 Dec 5 sale 16,700 71.00 Calculate the ending inventory balance for Oriole Ltd., assuming the company uses a perpetual inventory system and the first-in, first-out cost formula. Also calculate the per-unit cost of the last item sold. (Round unit costs to 2 decimal places, e.g. 52.75 and ending inventory to 0 decimal places, e.g. 5,276.)Ending inventory $Unit cost of the last item sold $ Why does the author use a question-and-answer format to develop the main points of the passage?A. to better show how different types of clocks compare and contrastB. to guide readers' understanding of a complex topicC. to more easily reveal the timeline of eventsD. to present a sequential order of events in an understandable way 60 papers cost $27. Find the cost of 16 papers. $0.72 The answer is not among the choices provided. $7.00 $7.25 O $72.00 $7.02