to what temperature do scientists need to raise a strand of mouse dna for it completely separate?

Answers

Answer 1

Denaturation is done at a very high temperature of 95 degree Celsius for complete separation of mouse DNA.

What is PCR?

The polymerase chain reaction, or PCR, is one of the most important uses of molecular biology. To amplify the target DNA molecule, PCR is utilised. In a thermal cycler, PCR is performed.

The three main stages to do PCR are as follows:

1. Denaturation 95 degrees Celsius is a very high temperature at which it is performed. This high temperature causes a process known as denaturation, which causes the DNA strands to split from one another.

2. The temperature is lowered to 55 degrees centigrade for renaturation in order to allow the denaturized DNA strands to anneal with one another.

3. DNA synthesis Deoxyribonucleotides, a thermostable DNA polymerase, and an RNA primer are added to the 3" ends of both strands to carry out the synthesis of new DNA strands relative to the template strands.

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Related Questions

suggest why an MRSA infection may have to be treated with many different antibiotics

Answers

Answer:

A trio of antibiotics that had become powerless against MRSA decades ago proved effective in infected mice when used together.

Although more testing is needed, the results suggest that combinations of already-approved antibiotics might add to our options to combat MRSA infections.

Explanation:

Are scallops decomposers?

Answers

Answer:

Yes they are.

Explanation:

also reminder to be happy drink water u are loved :). <3

Fill in the blank below with the vocabulary word that best completes the sentence.
Natural selection leads to
a process of change in a population over time.
DO NE

Answers

Answer:

natural growth

Explanation:

because all thing's which are natural are natural resources.

Answer:

Fill in the blank below with the word that best completes the sentence.

Natural selection leads to Evolution, a process of change in a population over time.

after

Complete the sentence below by selecting the correct words from the drop-down menus.

Factors that affect natural selection include

✔ variation

,

✔ overpopulation

, and

✔ adaptation

.

Explanation:

Which term means a volume of a certain rock type of a specific age?
x geologic map
geologic unit
contact
O fault line



The answer is geologic unit

Answers

Thanks. :) this is really :)

What is the relationship between atmospheric pressure and the density of gas particles in an area of decreasing pressure? O As air pressure in an area decreases, the density of the gas particles in that area decreases. O As air pressure in an area decreases, the density of the gas particles in that area increases. O As air pressure in an area decreases, the density of the gas particles in that area remains constant. O As air pressure in an area decreases, the density of the gas particles in that area increases and decreases in an alternating pattern.​

Answers

Answer:

As air pressure decreases so does density of gas particles

Explanation:


Why would a Nonsense mutation be less damaging if it occurred at the end of the gene rather than the
beginning?

Answers

Nonsense mutations make stop codons. If the mutation is towards the beginning of a gene, then the protein that gets created will be cut short and not function properly. But if it was towards the end, there is still a chance that the protein will still be able to work.

Someone please answer i’ll give you brainliest.

Answers

Answer:

You can go with first option

Explanation:

First option contains mitochondria, vacuole, nucleus, cell membrane, cytoplasm

These elements contained by both cells animals and plants

But in other option cell wall is also in the options

So, but it is contained by plant cells only so they are not correct

THANK YOU

If you are helped with my answer pls tag me brianliest I really want it.......

Rocks are made of collections of mineral grains that are held together in a firm, solid mass. Rocks are identified primarily by the minerals they contain, their color, and their texture.

Based on this passage, you can infer...

A. Rocks are classified into three major groups
B. Rocks form from the cooling and hardening of molten rock
C. A rock's origin is the way it was formed
D. Each type of rock has a distinctive set of minerals

OMG PLSSS HELP ME PLS PLS PLS!!!!!!!

Answers

Answer:

Explanation: it’s a

Which best helps scientists determine the age of fossil

Answers

Explanation:

To establish the age of a rock or a fossil, researchers use some type of clock to determine the date it was formed. Geologists commonly use radiometric dating methods, based on the natural radioactive decay of certain elements such as potassium and carbon, as reliable clocks to date ancient events.

The method that best helps scientists determine the age of fossils is radiometric dating. This method is highly accurate and reliable, providing precise numerical ages for fossils.

Radiometric dating is a technique that uses the decay of radioactive isotopes to calculate the absolute age of rocks, minerals, and fossils. Different isotopes have specific half-lives, which is the time it takes for half of the radioactive parent isotopes to decay into stable daughter isotopes.

By measuring the ratio of parent isotopes to daughter isotopes in a fossil or rock sample, scientists can determine how many half-lives have passed and calculate the age of the specimen.

This method is highly accurate and reliable, providing precise numerical ages for fossils and other geological materials, and helping scientists understand the timeline of Earth's history and the evolution of life on our planet.

Therefore, The method that best helps scientists determine the age of fossils is radiometric dating.

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what are the Seven parts of the water system

Answers

1: Evaporation

2: Condensation

3: Sublimation

4: Precipitation

5: Transpiration

6: Runoff

7: Infiltration

what is Spermatogenesis ?

Answers

Is the process by which haploid spermatozoa develop from germ cells in the seminiferous tubules of a testis

GIVING BRAINLIEST AND THE REST OF MY POINTS!!!!!!!

How does the location of the heart, lungs, and brain differ in the human body?

A) The brain and heart are located in the head while the lungs are located in the chest.
B) The brain is located in the head while the heart and lungs are located in the chest.
C) The heart is located in the head while the brain and lungs are located in the chest.
D) The lungs are located in the head while the heart and brain are located in the chest.

Answers

Answer:

B

Explanation:

The heart and lungs are in the chest, while the brain is located at the head.

Answer:

B

Explanation:

I got it right on my test

What kind of rock? Igneous, Metamorphic, sedimentary. Explain why? plz help!! ASAP

Answers

Answer:

sedimentary

Explanation:

it seems eagle rock has sedimentary features, such as layers, or ripple marks

What signals the end of
the cell cycle?
A. cell death
B. Cancer
C. Cell division

Answers

A, because after cell death there starts a new life for cells

Which organism is a secondary consumer?
a. Bear
b. Weasel
c. Deer
d. Rabbit
e. Grass

Answers

Answer:

I think weasel.

Hope it helps! :)

Explanation:

Answer:A.) Bear
Explanation:They eat other animals

The diagram shows a cladogram.
Which statement is supported by the cladogram?
A. The tuna does not have a
jaws.
B. The leopard does not walk on four legs.
C. The lamprey does not have vertebral column.
D. The salamander does not produce an amniotic egg.

Answers

Answer: C

Explanation:

which of the following is a matter

Answers

Answer:

gas, liquid and solid

Explanation:

hi

Matters are Liquids,Solids,Gasses:)))

please help!!!!!!!!!!!!!!!!!!

Answers

Answer:

The clownfish and anemone won't be able to survive in the wild without each other.  The clownfish provides the anemone with nutrients and scares off potential predator fish.  The anemone provides the clownfish with protection and shelter.

Explanation:

The clownfish won’t be able to survive

write two examples of consumers tell whether they are herbivores omnivores carnivors or scavengers

Answers

Answers

Lion,Tiger -Carnivores

vulture- scavenger

Cow,sheep -Herbivores

Man, Pig -Omnivores

A panda (herbivore) eats bamboo shoots and leaves, while a lion (carnivore) preys on other animals.A bear (omnivore) eats both plants and animals, while a vulture (scavenger) feeds on the remains of dead animals.What are consumers?

In an ecosystem, consumers are organisms that obtain their energy and nutrients by feeding on other organisms. They are a vital part of the food chain, and they come in different sizes and types.

Consumers can be classified into several categories based on what they eat. For example:

Herbivores: These are animals that consume only plants for their food. Examples include rabbits, cows, and giraffes.

Carnivores: These are animals that consume other animals for their food. Examples include lions, tigers, and sharks.

Omnivores: These are animals that eat both plants and animals. Examples include humans, bears, and raccoons.

Scavengers: These are animals that feed on the remains of dead organisms. Examples include vultures, hyenas, and some types of beetles.

Consumers play an important role in an ecosystem, as they help to transfer energy and nutrients from one level of the food chain to another. They are an integral part of maintaining the balance and stability of an ecosystem.

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Which of the following is not a tectonic boundary
A) convergent
B) divergent
C)transform
D) sedergent

Answers

Answer:

transform

Explanation:

trust me

what happens during cytokines

Answers

Answer:

during cytokines the cytoplasm( the liquid center of the cell that holds the organelles in to place) split into two equal half's, and the cell becomes two daughter cells.

Which cities are experiencing clear weather? Which are experiencing rainy weather?
(Help me with questions 3 and 4 in the attachment please :( )

Answers

Answer:

3.) San Fransico and Denver because they are far away from the rain

4.) Dallas because it is right next to the high pressure

How can you use what you have learned in Biology to help yourself, family and the planet?

Answers

Answer: I’m not sure what you’ve learned but for me it’s that you shouldnt leave the lights on when you don’t need them, you should switch off the TV when you aren’t using it, if you see a piece of trash pick it up and throw it in a trash can, and recycle

Explanation: HAPPY (late) EARTH DAY!!

If sperm contains both x and y chromosomes then who decides the gender. Whether egg chooses which sperm( a sperm carrying x or y chromosomes) has to be fertilized.

Answers

Answer:

Egg cells all contain an X chromosome, while sperm cells contain an X or Y chromosome.

Explanation:

This arrangement means that it is the male that determines the sex of the offspring when fertilizationocc

The process of one ecological community gradually changing into another is called:
community development
ecological succession
O community succession
O ecological development

Answers

Answer:

ecological succession

Phytoplankton are microscopic organisms that perform photosynthesis in oceans. When urban pollution runoff makes it to the oceans, it poses a threat to humans by killing numerous phytoplankton. How does reducing the number of phytoplankton most directly affect humans? O E. The atmosphere will contain more oxygen, resulting in warmer global temperatures. G. The atmosphere will contain more carbon dioxide, resulting in cooler global temperatures. H. The atmosphere will contain more oxygen, resulting in cooler global temperatures. O J. The atmosphere will contain more carbon dioxide, resulting in warmer global temperatures. WILL MARK BRAINLEST IF CORRECT!!!​

Answers

Answer:

The atmosphere will contain more oxygen, resulting in warmer global temperatures.

Explanation:

I did this on my test

The reduction of the number of phytoplankton that impact the human should be that atmosphere will contain more oxygen, leads in warmer global temperatures.

What is phytoplankton?

It is also called as the microalgae that should be same like the terrestrial plants which comprise of the chlorophyll and it needs the sunlight for living and growth.

It is to be considered as the buoyant and float in the upper part of the ocean where the sunlight should be penetrates with the water

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the ovary develops into the?

Answers

Answer:

Ovules

I hope this helps!

Answer:

fruit

Explanation:

The ovary itself will mature into a fruit, either dry or fleshy, enclosing the seeds

31. Which sequence shows increasing ecological
levels of organization?
A. organism, population, community, ecosystem
B. ecosystem, population, organism, community
C. community, ecosystem, population, organism
D. population, organism, ecosystem, community

Answers

Hello there, kawissaehsshen!!!

The answer here would be A.  The organism is a singular thing, so it should be first. The population comes next - it is all the same type of organism living in one ecosystem. Next is community - this is all the living organisms in the area. Lastly, there is the ecosystem - the organisms, their habitats, and everything else.

I really hope this helps you!!

During photosynthesis in plants, what is the source of the carbon in the glucose sugar molecule
(C6H12O6)?

Carbon dioxide in the air
Carbon monoxide in the air
Carbon particles in the soil
Carbon particles in water

Answers

Answer:

Carbon in the air

Explanation:

Write 5-6 sentences that explains roles of Heredity and probability.

Answers

Answer:

Probability is a method for determining the likelihood of something uncertain occurring. If you flip a coin, you do not know whether it will be heads or tails, but probability can tell you that there is a 1/2 chance of either happening. Probability is a method used to predict the likelihoods of uncertain outcomes. It is important for the field of genetics because it is used to reveal traits that are hidden in the genome by dominant alleles. Probability allows scientists and doctors to calculate the chance that offspring will inherit certain traits, including some genetic diseases like cystic fibrosis and Huntington's disease.

Explanation:

Other Questions
Please someone help me (NO LINKS) (NO LYING) 3 CO2How many atoms of Oxygen (O) are there? Please help meThink about what the narrator learns about the Wonder Horse.Does he have to capture the Wonder Horse to learn these things? Why or why not? Sorry for the lack of questions yesterday! Heres another one, though:What are the next three letters in this combination? OTTFFSS The ___________________ of cities created the need for public parksa.colonizationc.urbanizationb.irrigationd.All of the above b) How does adaptation affect the survival of a species? Why? What are the duties of the attorney general? Check all that apply. defending the laws of the state in court being responsible for the custody of state funds keeping the Great Seal of the State of Louisiana serving as the head of the Department of Justice advising or assisting on criminal cases as needed serving as an ex officio member on certain committees which of the following is true about conflict? A. conflict can sometimes occur for no reason at allB. Conflict is inevitable C. Conflict can be avoided in any situationD. Conflict can rarely be prevented by critical thinking Please help Ill mark you as brainliest if correct!ABCD 1) Eight less than triple a number ___________________ 2) The product of a number and fifteen decreased by six __________________3) Lily has five more dollars than the money that her brother has. Her brother has d dollars.a. Write an expression that represents the amount of money Lily has. b. If her brother has $4.50, how much money does Lily have? People think my favorite color is black.It's really more of a deep, deep blue for me.Like the color of the sky just after sunset and before night, but also like the sky just before the sun is about to rise. Death and rebirth.Death is my dominion, they say, but it is rebirth, too, that I dominate. My other is growth.Persistent, but weak.I can't blame her. They believe that I think life is a joke.Perhaps it is.I want to laugh about it, but I can't bring myself to do it. Life is... tragic.Humans, especially.Because they know it better than other animals.They wish there was a hope beyond me, because I ruin everything for them.I do. I wish I couldn't, but there's no other way. My job will not be done until everything that breathes and walks is gone from the earth.So what can I do?Nothing.Just like the tragedy of life.I will sit here, and wait. Until nothing is left. 5. Malnutrition, especially getting too little protein to eat, can cause muscles to waste away.false true Strands of DNA are tightly coiled in A. DNA B. Base pairs C. Chromosomes D. Mitochondria pls help 10 free points :]Activity log - Isolating the Lower BodyRecord your time and activity below and in your unit fitness log. If a particular category does not apply, leave it blank.Date: Warm-up: Cool-down: Type of Activity: (Cardiovascular, Muscular, or Flexibility) Description of Activity and Time: Intensity Level: (Moderate or Vigorous) Total Time: 7Which statement best explains the relationship between specialization and the seafood industry in Louisiana?A The seafood industry imports large amounts of seafood in order to pay fewer taxes.B Producers are able to focus on high-quality seafood to compete with imports.The seafood industry has a limited supply of different types of seafood.Consumers are unable to purchase many types of high-quality seafood. These cultural ideal did not apply to everyone in the United States, however. List four groups that were often denied equal opportunities in the years following the Revolutionary War. Someone pls help (Beccas dog walking business started with 1 dog. She walks 2 additional dogs each month.The graph represents the problem. How many dogs does she walk in the third month?) Why is this article of the Constitution called "The Supremacy Clause?""This Constitution, and the Laws of the United States which shall be made in pursuance thereof; and all treaties made, or which shall be made, under the authority of the United States, shall be the supreme law of the land; and the judges in every state shall be bound thereby, anything in the constitution or laws of any state to the contrary notwithstanding." - Article IV, Section 2 of the US ConstitutionA. The Constitution is the law of the land and no law can conflict with it.B. Laws and statutes passed by the United Nations overrule the U.S. Constitution.C. All state constitutions are the law of the land and no law can conflict with it.D. The US promised to pay all debts and honor all agreements. Which material transmits the most visible light?A.Sunglass lensB.MirrorC.Clear glassD.Black cloth A rectangle swimming pool is measured up to 15 meters long and 7 1 fourth meters wide what is the area of this swiming pool